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rabbit anti na v 1 5  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti na v 1 5
    Rabbit Anti Na V 1 5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+na+v+1+5/Anti-NaV1%2E5+(SCN5A)+(493-511)+Antibody/pmc12785957-182-3-7
    Average 96 stars, based on 137 article reviews
    rabbit anti na v 1 5 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Aqueous Normal-phase Chromatography:

    Article Title: Inflammatory Co-Regulation of Voltage-Gated Sodium Channels and Na,K-ATPase in Metastatic Breast Cancer
    Article Snippet: .. Primary antibodies were rabbit anti-Na v 1.5 (Alamone Labs, ASC-005, 1:400), guinea pig anti-Na v 1.5 (Alamone Labs, ASC-005-GP, 1:400), anti-Na,K-ATPase α1 (Alamone Labs, ANP-001, 1:200), anti-Na,K-ATPase α3 (Alamone Labs, ANP-003, 1:200), and anti-PCM-1 (Cell Signaling Technology, Danvers, MA, USA, 5213, 1:200). .. Secondary antibody Alexafluor 488 anti-rabbit (Invitrogen, A-11008, 1:1200) or Alexafluor 594 anti-guinea pig (Invitrogen, A-11076, 1:1200) diluted in blocking buffer was incubated for 45 min at room temperature.

    Incubation:

    Article Title: Na V 1.5 knockout in iPSCs: a novel approach to study Na V 1.5 variants in a human cardiomyocyte environment
    Article Snippet: They were resolved on 4–12% gradient stain-free SDS–polyacrylamide gels (Bio-Rad) and were blotted on 0.45-μm PVDF membranes (BioRad). .. The membranes were blocked and were incubated with rabbit anti-Na V 1.5 (1:200, Cat #ASC-005, Alomone Labs, Israel) or anti-Pan Na V antibodies (1:200, Cat #ASC-003, Alomone Labs, Israel) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:3000, Cat #2118, Cell Signaling Technology, USA). .. Horseradish peroxidase-conjugated anti-rabbit (1/3000, Cat #7074, Cell Signaling Technology) was used as secondary antibody.

    Article Title: The Na V 1.5 auxiliary subunit FGF13 modulates channels by regulating membrane cholesterol independent of channel binding
    Article Snippet: Samples were analyzed by SDS-PAGE on an 8%–16% Tris-glycine gel transferred to a PVDF membrane using iBlot3 (Thermo Fisher Scientific). .. Membranes were blocked in blocking buffer (3% BSA [m/v] and 0.1% Tween 20 [v/v]) for 1 hour at room temperature and incubated overnight with primary antibodies diluted in blocking buffer at 4°C: rabbit anti-Na V 1.5 (Alomone; 493-511) 1:1,000; custom rabbit anti-FGF13 antibody, previously described ( ); and mouse anti-GAPDH (Thermo Fisher Scientific; MA5-15738) 1:1,000. .. Membranes were washed 5 times in TBST washing solution (150 mM NaCl, 20 mM Tris, pH 7.6, and 0.1% Tween 20 [v/v]) and incubated with secondary antibodies diluted in blocking buffer for 1 hour at room temperature: goat anti-rabbit IgG, HRP-linked (Cell Signaling Technology; 7074) 1:5,000 and anti-mouse m-IgGκ BP-HRP (Santa Cruz Biotechnology; sc-516102).

    Blocking Assay:

    Article Title: The Na V 1.5 auxiliary subunit FGF13 modulates channels by regulating membrane cholesterol independent of channel binding
    Article Snippet: Samples were analyzed by SDS-PAGE on an 8%–16% Tris-glycine gel transferred to a PVDF membrane using iBlot3 (Thermo Fisher Scientific). .. Membranes were blocked in blocking buffer (3% BSA [m/v] and 0.1% Tween 20 [v/v]) for 1 hour at room temperature and incubated overnight with primary antibodies diluted in blocking buffer at 4°C: rabbit anti-Na V 1.5 (Alomone; 493-511) 1:1,000; custom rabbit anti-FGF13 antibody, previously described ( ); and mouse anti-GAPDH (Thermo Fisher Scientific; MA5-15738) 1:1,000. .. Membranes were washed 5 times in TBST washing solution (150 mM NaCl, 20 mM Tris, pH 7.6, and 0.1% Tween 20 [v/v]) and incubated with secondary antibodies diluted in blocking buffer for 1 hour at room temperature: goat anti-rabbit IgG, HRP-linked (Cell Signaling Technology; 7074) 1:5,000 and anti-mouse m-IgGκ BP-HRP (Santa Cruz Biotechnology; sc-516102).



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